Identification of an ancillary protein, YabF, required for activity of theKefC glutathione-gated potassium efflux system in Escherichia coli

Authors
Miller, S Ness, LS Wood, CM Fox, BC Booth, IR
Citation
S. Miller et al., Identification of an ancillary protein, YabF, required for activity of theKefC glutathione-gated potassium efflux system in Escherichia coli, J BACT, 182(22), 2000, pp. 6536-6540
Citations number
26
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
00219193 → ACNP
Volume
182
Issue
22
Year of publication
2000
Pages
6536 - 6540
Database
ISI
SICI code
0021-9193(200011)182:22<6536:IOAAPY>2.0.ZU;2-L
Abstract
A new subunit, YabF, for the KefC K+ efflux system in Escherichia call has been identified. The subunit is required for maximum activity of KefC. Dele tion of yabF reduces KefC activity 10-fold, and supply of YabF in trans res tores activity. IS2 and IS10R insertions in yabF can be isolated as suppres sors of KefC activity consequent upon the V427A and D264A KefC mutations.