Liquid chromatographic-tandem mass spectrometric method for the analysis of a neurokinin-1 antagonist and its metabolite using automated solid-phase sample preparation and automated data handling and reporting

Pharmacokinetic studies play a vital role during the development of new pha rmaceutical substances. Data presented demonstrate an accurate, precise and robust assay for a neurokinin-1 receptor antagonist and its metabolite wit h HPLC-MS-MS. Sample preparation is performed by solid-phase extraction (SP E) in the 96-well plate format. This process is fully automated with a Teca n Genesis(TM) pipetting system using its standard robotic manipulator arm ( ROMA). All instruments are fully integrated in a study oriented laboratory information system (LIMS) with an Oracle database that communicates bi-dire ctional with the analytical equipment. Finally, the results are reported by push button operation. (C) 2000 Elsevier Science B.V. All rights reserved.