The anatomy of third molars has been described as unpredictable. However re
storative, prosthetic, and orthodontic considerations often require endodon
tic treatment of third molars in order for them to be retained as functiona
l components of the dental arch. The purpose of this study was to investiga
te and characterize the anatomy of maxillary and mandibular third molars. O
ne hundred fifty maxillary and 150 mandibular extracted third molars were v
acuum-injected with dye, decalcified, and made transparent. The anatomy of
the root canal system was then recorded. Seventeen percent of mandibular mo
lars had one root (40% of which contained two canals), 77% had two roots, 5
% had three roots, and 1% had four roots. Teeth with two roots exhibited hi
ghly variable canal morphology, containing from one to six canals, includin
g 2.2% that were "C-shaped" Fifteen percent of maxillary molars had one roo
t, 32% had two roots, 45% had three roots, and 7% had four roots. Teeth wit
h one root demonstrated the most unusual morphology, with the number of can
als varying from one to six. An in vivo study of the canal morphology of tr
eated third molars is suggested to provide the practitioner with an underst
anding of the clinical implications of third molar root anatomy.