Evaluation of two assays, MSRV and RV, for the isolation of Salmonella spp. from wastewater samples and broiler chickens

The Rappaport Vassiliadis (RV) and the Modified Semi-solid Rappaport Vassil iadis (MSRV) methods were evaluated in 130 municipal wastewater samples and 30 flocks of broilers. Analysis of the results showed that 71 Salmonella s erotypes were isolated in wastewater samples. The positivity of the MSRV me thod was 33% and of the RV method, 45%. The sensitivity was 95% and 83% wit h the MSRV and RV, respectively. The concordance between the two methods wa s 89% (k = 0785). One hundred serotypes were isolated from broiler internal organs and 50 from intestinal samples. For internal organs, the positivity of MSRV was 56% and of RV, 45%. For intestinal samples, the respective per centages were 28 and 22. For internal organs, the sensitivity was 100% with MSRV and 81% with RV, whereas for intestinal samples, the sensitivity was 100% with MSRV and 80% with RV. The specificity was 100% in all cases. The concordance between the two methods was 89% (k = 0790) for internal organs and 94% (k = 0851) for intestinal samples.