T. Nagase et al., Differential expression of two glucuronyltransferases synthesizing HNK-1 carbohydrate epitope in the sublineages of the rat myogenic progenitors, MECH DEVEL, 98(1-2), 2000, pp. 145-149
HNK-1 epitope is a cell-surface carbohydrate mediating various cell-cell or
cell-substrate interactions. We found HNK-1 epitope in longitudinally arra
yed fibers in the subpopulation of the epaxial myotome, and hypaxial myobla
sts migrating into the limb bud in the rat embryo. We next investigated the
expression patterns of genes encoding two glucoronyltransferases (GlcAT-P,
GlcAT-D) and sulfotransferase (Sul-T), which are required for biosynthesis
of HNK-1 epitope. GBcAT-P gene was expressed in the non-migrating longitud
inal fibers, whereas GlcAT-D gene was expressed in the migrating myoblasts
in the limb bud, Sul-T gene expression was ubiquitously observed in all the
se myogenic populations. Thus, differential expression of GlcAT genes may r
elate to the epaxial/hypaxial or migrating/non-migrating myoblast lineages.
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