Caspase-3 activation by beta-amyloid and prion protein peptides is independent from their neurotoxic effect

Synthetic peptides corresponding to residues 25-35 of beta -amyloid (beta 2 5-35) and 106-126 of prion protein (PrP 106-126) are amyloidogenic and caus e neuronal death by apoptosis in vitro. We evaluated, in rat cortical neuro ns, the role of caspases activation in the peptides neurotoxicity by measur ing of caspase-3 (CPP32) activity and applying a non-selective caspase inhi bitor (z-VAD-fmk) or CPP32-specific inhibitor (Asp-Glu-Val-Asp-CHO (DEVD-CH O)). CPP32 was dose-dependently activated by both peptides (2.5-50 muM). Th e caspase inhibitors completely abolished the CPP32 activation induced by t he peptides. However, the neurotoxic effect was partially attenuated with z -VAD-fmk, while no antagonism was found with DEVD-CHO. Thus, although beta 25-35 and PrP 106-126 robustly activated CPP32, their neurotoxic effect was independent of this caspase activation. (C) 2000 Published by Elsevier Sci ence Ireland Ltd.