Fluorescent labelling of oligodeoxyribonucleotides by the oxyamino-aldehyde coupling reaction

Authors
Trevisiol, E Renard, A Defrancq, E Lhomme, J
Citation
E. Trevisiol et al., Fluorescent labelling of oligodeoxyribonucleotides by the oxyamino-aldehyde coupling reaction, NUCLEOS NUC, 19(9), 2000, pp. 1427-1439
Citations number
15
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
19
Issue
9
Year of publication
2000
Pages
1427 - 1439
Database
ISI
SICI code
1525-7770(2000)19:9<1427:FLOOBT>2.0.ZU;2-Q
Abstract
We describe the reaction of oligonucleotides containing an aldehydic group at the 5'-end or inside the sequence with an oxyamino label. The reaction w as found to be highly selective and represents an efficient method for deri vatization of oligonucleotides.