M. Kowalczyk et al., Nonlinear delayed fluorescence in confocal scanning microscopy. Point-spread-function analysis, OPT APPL, 30(2-3), 2000, pp. 231-238
Generation of the signal beam in the confocal scanning fluorescence microsc
opy by means of nonlinear delayed fluorescence phenomenon based on triplet-
triplet annihilation is proposed. From the point of view of the spatial res
olution of three-dimensional confocal imaging the superiority of the phenom
enon proposed here over the classical two-photon fluorescence is demonstrat
ed. This is done in terms of axial and lateral point spread functions compu
ted for the assumed model of nonlinearity.