This paper summarizes our recent work on the regulation and expression
of a gene family encoding a vegetative homologue (Blec) of the pea se
ed lectin. Blec is encoded by a multigene family whose members share 4
0-50% identity with other legume lectins. Blec RNA is highly expressed
within specific phases of epidermal cell development, it accumulates
specifically in meristematic epidermal cells of vegetative and floral
organs, but is undetectable in protodermal cells that bound vegetative
or floral meristems. Furthermore, during early vegetative and floral
primordia development Blec accumulates mainly on the abaxial epidermis
and is undetectable on the adaxial or inner surface of such organs. T
he accumulation of Blec thereby not only defines the epidermis, but al
so specific stages and positions of epidermal tissue during organ deve
lopment. These observations are discussed with a view to further defin
ing the regulatory events involved in epidermal differentiation. As a
first step, a Blec genomic clone has been expressed in alfalfa RegenSY
. Preliminary analysis shows that, as in pea, Blec is expressed in alf
alfa RegenSY in an apex specific manner.