Ve. Gould et al., A novel, nuclear pore-associated, widely distributed molecule overexpressed in oncogenesis and development, AM J PATH, 157(5), 2000, pp. 1605-1613
Citations number
37
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
Nuclear pore complexes are large, elaborate macromolecular structures that
mediate the bidirectional nucleocytoplasmic traffic. In vertebrates, nuclea
r pore complexes comprise 50 to 100 proteins termed nucleoporins (Nup). An
88-kd nucleoporin (Nup88) has been recently cloned and characterized, and f
ound to be associated in a dynamic subcomplex with the oncogenic nucleopori
n CAN/Nup 214. We have produced a polyclonal antiserum to Nup88, and found
that it immunoreacts convincingly in conventional tissue sections of 214 sa
mples of malignant tumors of many types. All carcinomas were stained irresp
ective of site or line of differentiation; the majority of cases reacted st
rongly and extensively, In situ carcinomas and highly dysplastic epithelia
were similarly reactive. Samples of malignant mesotheliomas, gliomas, sarco
mas, and lymphoreticular tumors were also stained. Substantial reactions we
re also found in certain fetal tissues, Focal reactions were noted in some
reactive-proliferative processes. Most benign epithelial and mesenchymal tu
mors and hyperplasias, and normal adult tissues reacted weakly and sporadic
ally or not at all. Immunoblot analysis of selected samples strongly corrob
orated those findings. If further substantiated, our findings indicate that
Nup88 could be regarded as a selective yet broadly based proliferation mar
ker of potential significance in the histological evaluation and diagnosis
of malignant transformation. Its ready applicability on conventional paraff
in sections acid on cytological preparations may broaden its clinical and i
nvestigative significance.