A fluorescent-labeled oligopeptide for monitoring PKA-mediated phosphorylation

Authors
Ohuchi, Y Katayama, Y Maeda, M
Citation
Y. Ohuchi et al., A fluorescent-labeled oligopeptide for monitoring PKA-mediated phosphorylation, ANALYST, 125(11), 2000, pp. 1905-1907
Citations number
8
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYST
ISSN journal
00032654 → ACNP
Volume
125
Issue
11
Year of publication
2000
Pages
1905 - 1907
Database
ISI
SICI code
0003-2654(2000)125:11<1905:AFOFMP>2.0.ZU;2-O
Abstract
A fluorescent-labeled oligopeptide (DACM-CLRRASLK-fluorescein), containing a consensus amino acid sequence (RRXSL) of cyclic AMP (cAMP) dependent prot ein kinase A (PKA) substrate-proteins, was designed. The fluorescent peptid e was a good substrate of PKA, and the phosphorylation of its serin residue caused an intensive change in fluorescent intensity. We expect that the pe ptide will be useful as a fluorescent indicator for monitoring PKA activity in living cells.