Description and validation of an analytical method for the determination of paromomycin sulfate in medicated animal feeds

A procedure for the extraction of paromomycin from different animal feed ma trices (rabbit, chicken, pig feeds) and its subsequent determination via a reversed-phase ion-pair HPLC separation coupled with pulsed amperometric de tection is described. The procedure optimised in terms of the extracting so lvent and the solid phase extraction stationary phase allows the total reco very of the aminoglycoside antibiotic. The criteria used for the validation of the analytical method applied to the cited matrices are the linear dyna mic range of the response, the detection limit, the repeatability, the inte rmediate repeatability and the accuracy. A comparison with a method describ ed in the literature for the bulk analysis of this antibiotic is made.