Heterologous amplification of microsatellite loci from mouse and rat in oryzomyine and thomasomyine South American rodents

Citation
Cav. Lima-rosa et al., Heterologous amplification of microsatellite loci from mouse and rat in oryzomyine and thomasomyine South American rodents, BIOCHEM GEN, 38(3-4), 2000, pp. 97-108
Citations number
43
Categorie Soggetti
Molecular Biology & Genetics
Journal title
BIOCHEMICAL GENETICS
ISSN journal
00062928 → ACNP
Volume
38
Issue
3-4
Year of publication
2000
Pages
97 - 108
Database
ISI
SICI code
0006-2928(200004)38:3-4<97:HAOMLF>2.0.ZU;2-4
Abstract
Eleven heterologous primers were investigated in 119 individuals of II spec ies of rodents of the Oryzomyine and Thomasomyine groups. The animals were collected at four sites of the "Cerrado, " a dryland biome located on the B razilian Plateau, all of them being karyotyped and taxonomically allocated according to the karyotype. Four of these primers, R47, R65, R75 (from Ratt us), and ATP (from Mus) cross-amplified in at least one of these taxa, givi ng products of seven, nine, one, and three bands, respectively. These value s are of the same order as others obtained when heterologous primers were a mplified in other orders of mammals. Of the 20 products amplified in these two rodent groups by these four primers, only 7 of the bands were seen in a heteromorphic stare tone individual presenting two bands), in two species (Rhipidomys aff. leucodactylus and Oryzomys megacephalus). The others occur red as monomorphic bands.