CHARACTERISTICS OF HYBRIDS USING PROTOPLA STS IN PLEUROTUS-CORNUCOPIAE (PAULET) ROLLAND VAR CITRINOPILEATUS

We isolated four types of monokaryotic mycelia by the protoplast isola tion method from two strains of the basidiomycete Pleurotus cornucopia e (Paulet) Rolland var, citrinopileatus which were designated as ABm ( our laboratory's stock Pc71-1 with constituted nuclei A, B, and cytopl asm m) and CDn (our laboratory's stock Pc76-5 with constituted nuclei C, D, and cytoplasm n). Using these mycelia for crossing, we obtained eight dikaryotic mycelia and four monokaryotic mycelia with exchanged cytoplasm (An, Bn, Cm and Dm), These monokaryotic mycelia were obtaine d from the protoplast regeneration from these dikaryotic mycelia. All of the strains described above were examined to clarify the mycelial g rowth rates at various temperatures, the calculated optimum growth tem perature (thm), and the suitable growth temperature range (thw). In ad dition, all of the dikaryotic mycelia were examined to clarify the cul tural and morphological characteristics. As a result, certain nuclei a ffected these characteristics. Nuclei C influenced the mycelial growth rate, thw, primordium formation, and size and color of fruit bodies. Nuclei B controlled the yield of fruit bodies. Furthermore, cytoplasm did not affect thm, thw, and the cultural and morphological characteri stics. However, cytoplasm influenced the myclial growth rate of certai n dikaryotic mycelia at low culutivation temperatures.