D. O'Beirne et G. Hamer, The utilisation of glucose/acetate mixtures by Escherichia coli W3110 under aerobic growth conditions, BIOPROC ENG, 23(4), 2000, pp. 375-380
Byproduct acetate is of major concern when considering the growth of Escher
ichia coli on glucose. Besides the fact that acetate production detracts fr
om the overall yield, acetate itself is also a growth inhibitor. To further
complicate matters, E. coli is capable of growth on acetate via the glyoxy
late bypass. In an effort to evaluate the influence of acetate on the growt
h of E. coli, mixed glucose/acetate substrate batch and continuous cultivat
ions have been carried out. The results have been examined in the context o
f their implications for industrial-scale bioreactors. It was found that ac
etate was growth inhibitory at relatively low concentrations <1.25 g/l. A b
atch culture supplied with both glucose and acetate utilised glucose in pre
ference to acetate. Simultaneous utilisation of substrates was observed in
continuous cultures at low imposed growth rates, i.e., below ca. 0.30 h(-1)
.