Activity and stability of immobilised soybean lipoxygenase-1 in aqueous and supercritical carbon dioxide media

Authors
Chikere, AC Galunsky, B Overmeyer, A Brunner, G Kasche, V
Citation
Ac. Chikere et al., Activity and stability of immobilised soybean lipoxygenase-1 in aqueous and supercritical carbon dioxide media, BIOTECH LET, 22(22), 2000, pp. 1815-1821
Citations number
34
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
22
Year of publication
2000
Pages
1815 - 1821
Database
ISI
SICI code
0141-5492(200011)22:22<1815:AASOIS>2.0.ZU;2-I
Abstract
The activity of immobilised soybean lipoxygenase-1 (LOX-1) was studied in a queous and supercritical carbon dioxide (SCCO2) media for the production of 13S-hydroperoxyoctadecadenoic acid (13S-HPODE). In SCCO2, it was optimal a t 33 degreesC and 25 MPa. A higher space-time yield of 5.7x10(-3) Ms-1 mg(- 1) LOX-1 for 13S-HPODE was obtained in SCCO2 compared to only 5x10(-5) Ms-1 mg(-1) LOX-1 in aqueous medium. The stability of immobilised LOX-1 was onl y significantly affected by the pressurisation and depressurisation steps d uring reactions in SCCO2.