Epitope analysis of a rice allergen with a monoclonal antibody secreted bya human-mouse hybridoma

Authors
Shinmoto, H Yamagishi, K Kimura, T Suzuki, M
Citation
H. Shinmoto et al., Epitope analysis of a rice allergen with a monoclonal antibody secreted bya human-mouse hybridoma, BIOTECH LET, 22(22), 2000, pp. 1823-1826
Citations number
14
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
22
Year of publication
2000
Pages
1823 - 1826
Database
ISI
SICI code
0141-5492(200011)22:22<1823:EAOARA>2.0.ZU;2-U
Abstract
Five human human-mouse hybridomas secreting human monoclonal antibodies to rice allergens were established. Antibodies secreted from the hybridomas re acted with 14-16 kDa rice major allergen proteins. Analysis with overlappin g peptides synthesized on a multi-pin apparatus revealed a binding sequence of the major rice allergen protein RA17. The antigenic determinant was loc ated in the C-terminus region of the RA17 protein.