Citation
Zq. Zhang et Zg. Su, Single step Al2O3 chromatography for improved separation and recovery of Taxol, BIOTECH LET, 22(21), 2000, pp. 1657-1660
Citations number
7
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492
→ ACNP
Volume
22
Issue
21
Year of publication
2000
Pages
1657 - 1660
Database
ISI
SICI code
0141-5492(200011)22:21<1657:SSACFI>2.0.ZU;2-O
Abstract
An improved method that uses a single alkaline Al2O3 chromatography step wa
s developed both for separating Taxol from the extracts of Taxus cuspidate
callus cultures, and meanwhile converting other taxanes to Taxol catalyzed
by the Al2O3 adsorbent. Under the optimized operating conditions, a Taxol r
ecovery of 170% was obtained. The final Taxol content was 29% starting from
less than 1% in the initial extracts.