A solid phase IgM-capture radioimmunoassay (MACRIA) for the detection
of parvovirus B19-IgM is described (Cohen et al., 1983, J. Hyg. Camb.
91, 113-130). IgM from a dilution of patients serum is 'captured' onto
a solid phase coated by anti-human IgM. To determine whether any of t
he IgM is specific for parvovirus B19, B19 antigen is added followed b
y a detector system. In the MACRIA described here the detector system
comprises a mouse monoclonal antibody to parvovirus B19 and a I-125-la
belled anti-mouse antibody. A calibration curve derived from a standar
d B19 IgM serum is used to quantify B19 IgM using a single dilution of
test sera. The purpose of the protocol is the diagnosis of recent acu
te infection with parvovirus B19. (C) 1997 Elsevier Science B.V.