AN EVALUATION OF 9 COMMERCIAL EIA-KITS FOR THE DETECTION OF MEASLES SPECIFIC IGG

Nine commercial EIAs for measles-specific IgG were compared with haema gglutination inhibition (HI) and plaque reduction neutralization (PRN) . A total of 174 sera selected: to give approximately half of the sera without measles antibody by HI, were tested by all EIAs and HI. Hoewe ver, there was sufficient volume of only 101 samples for testing by PR N. A dilution curve of the British Standard measles antibody serum was also tested by each EIA. Assays were evaluated qualitatively against a consensus EIA result, HI and PRN: Gull, Melotest and Behring EIAs pe rformed best. Quantitative evaluation was by assessment of the charact eristics of the standard dilution curve, and by plotting differences w ith PRN against mean: Gull and Melotest EIAs were best. (C) 1997 Elsev ier Science B.V.