EFFICIENT TRANSCRIPTION OBTAINED BY A NEW PROCEDURE OF THE TRANSFER-RNA-LIKE STRUCTURE OF TURNIP-YELLOW-MOSAIC-VIRUS BY A TEMPLATE-DEPENDENT AND SPECIFIC VIRAL-RNA-POLYMERASE (VOL 64, PG 181, 1997)

The RNA-dependent RNA polymerase (RdRp) of turnip yellow mosaic virus (TYMV) was isolated by a simple, new method. An active, template-depen dent and specific enzyme was obtained. Although the genomic RNA of TYM V could not be transcribed completely during an invitro RdRp assay, a complete double-stranded product was identified as being of negative p olarity by complete digestion with ribonuclease T1. Antibodies directe d to part of the N-terminal (Ab140) or C-terminal (Ab66) invitro autoc leavage products of the large non-structural polyprotein of TYMV, coul d both partially inhibit RdRp activity peaks with different protein co mpositions. Both peak fractions retained high specificity for transcri ption of TYMV RNA. A protein of approximately 115 kDa was detected by both Ab140 and Ab66.