The influence of simple sugars, salts, and Botrytis-specific monoclonal antibodies on the binding of bacteria and yeast to germlings of Botrytis cinerea

The influence of simple sugars, salts, and Botrytis-specific monoclonal ant ibodies on the binding of three bacteria (Enterobacter aerogenes Hormaeche & Edwards, Enterobacter cloacae (Jordan) Hormaeche & Edwards, and Ochrobact rum anthropii gen.nov.) and three yeasts (Candida sake (Sarto & Ota) van Ud en & Buckley, Candida pulcherrima (Lindner) Windisch, and Trichosporon pull ulans (Lindner) Diddens & Lodder) to Botrytis cinerea (Persoon:Fr) was exam ined. Solutions of 0.1 M D(+)-galactose, L-fucose, or Botrytis-specific mon oclonal antibodies significantly reduced populations of E. aerogenes and E. cloacae adhering to pathogen germlings, whereas 0.1 M raffinose significan tly reduced C. sake or C. pulcherrima adhesion. In cytochemical studies, le ctin-gold labeling demonstrated the presence of galactose moieties in the w alls or matrix of E. aerogenes, and this labeling was diminished in bacteri a that were attached to B. cinerea. Immunolabeling with a Botrytis-specific monoclonal antibody that recognizes a glycoprotein was particularly intens e in condensed regions of the pathogen matrix associated with adherent E. a erogenes, whereas C. sake - B. cinerea interactions revealed a loose encaps ulation of adherent yeast cells by the matrix of B. cinerea. Results from t his study suggest the presence of several adhesion mechanisms that can be d istinguished according to yeast or bacterial binding and further defined ac cording to the genus.