Losses of heterozygosity in endometrial adenocarcinomas: Positive correlations with histopathological parameters

We analyzed 37 samples of endometrial adenocarcinoma for loss of heterozygo sity (LOH) by using a panel of 44 microsatellites located in 29 chromosomal regions. The aim of our study was to investigate the existence of a possib le preferential involvement of some tumor suppressor genes in endometrial c arcinogenesis. The analysis was performed on tumoral tissue and on a corres ponding normal tissue by the use of polymerase chain reaction (PCR) and the comparison of the amplified alleles. We observed significative LOH (>20%) in the chromosomal regions of 2q14 (33.33%), 7q35 (24.00%), 10q22.1 (37.50% ), 11q13-q14 (44.12%), 15q26 (40.63%), 17p13 (25.71%), and 17q21.3 (37.04%) . We defined a 1-cM minimal common deletion in 11q13-q14 between D11S911 an d D11S937 markers. A statistical analysis revealed a positive correlation b etween LOH of 11q13-q14 and clinicopathological data. (C) 2000 Elsevier Sci ence Inc. All rights reserved.