Chromosomal localization of a novel retinoic acid induced gene RA28 and protein distribution of its encoded protein

Gene RA28 is a retinoic acid induced novel gene isolated in our laboratory previously. All-trans retinoic acid (ATRA) was used to induce lung adenocar cinoma cell line GLC-82, and RA28 was obtained by subtractive hybridization . Green fluorescent protein (GFP) has emerged as a unique tool for examinin g introcellular phenomena in living cells. GFP possesses an intrinsic fluor escence at 488 nm that does not require other co-factors. In this report, a n eukaryotic expression plasmid pEGFP-C1-RA28 was constructed and transfect ed with parental cell line GLC-82 to analyze protein expression and its dis tribution in living cells. Moreover, radiation hybrid (RH) technique was us ed to localize RA28 to the chromosome. The results show that gene RA28 is m apped to the chromosome 19q13.1 region, its encoded protein is distributed on cell membrane. All the results further demonstrate that GFP and RH techn iques are accurate, fast, repetitive, and will be powerful methods for inve stigating the gene and protein localization.