Microsatellite DNA analysis proves nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda

Authors
Ding, B Shi, P Xiangyu, JG Zhang, YP Chen, DY Sun, QY Li, GP Wang, MK Liu, YL Kang, L Han, ZM Song, XF Li, JS Chen, YC
Citation
B. Ding et al., Microsatellite DNA analysis proves nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda, CHIN SCI B, 45(20), 2000, pp. 1883-1885
Citations number
7
Categorie Soggetti
Multidisciplinary
Journal title
CHINESE SCIENCE BULLETIN
ISSN journal
10016538 → ACNP
Volume
45
Issue
20
Year of publication
2000
Pages
1883 - 1885
Database
ISI
SICI code
1001-6538(200010)45:20<1883:MDAPNO>2.0.ZU;2-E
Abstract
A method for DNA isolation from early development of blastocyst and further analysis of nuclear and mitochondrial DNA was developed in present study. Total DNA was prepared from interspecies reconstructed blastocyst and a gia nt panda specific microsatellite locus g(010) was successfully amplified. D NA sequencing of the PCR product showed that two sequences of reconstructed blastocysts are the same as that of positive control giant panda. Our resu lts prove that the nucleus of interspecies reconstructed blastocyst comes f rom somatic nucleus of donor giant panda.