Complete nucleotide sequence and genomic organization of Periplaneta fuliginosa densonucleosis virus

We have cloned the replicative form of the Periplaneta fuliginosa densonucl eosis virus (PfDNV) genome and determined its complete sequence. The sequen ce has 5 454 nucleotides (nt), the genome consists of an internal unique se quence flanked by inverted terminal repeats (201 nt). The first 122 nt at t he 5' end and the terminal 122 nt at the 3' end of both plus and minus stra nds can fold into a typical hairpin structure. The genome contains seven ma jor open reading frames (ORFs). The plus strand has 4 ORFs occupying the 5' half of the plus strand, whereas the others span the 5' half of the minus strand. Two potential promoters were found at map units (m.u.) 3 and 97. Co mputer analysis of sequence homologies with other parvoviruses suggests tha t the plus strand of Pf DNV encodes very likely the nonstructural proteins and the minus strand probably encodes the structural proteins.