We have cloned the replicative form of the Periplaneta fuliginosa densonucl
eosis virus (PfDNV) genome and determined its complete sequence. The sequen
ce has 5 454 nucleotides (nt), the genome consists of an internal unique se
quence flanked by inverted terminal repeats (201 nt). The first 122 nt at t
he 5' end and the terminal 122 nt at the 3' end of both plus and minus stra
nds can fold into a typical hairpin structure. The genome contains seven ma
jor open reading frames (ORFs). The plus strand has 4 ORFs occupying the 5'
half of the plus strand, whereas the others span the 5' half of the minus
strand. Two potential promoters were found at map units (m.u.) 3 and 97. Co
mputer analysis of sequence homologies with other parvoviruses suggests tha
t the plus strand of Pf DNV encodes very likely the nonstructural proteins
and the minus strand probably encodes the structural proteins.