Electrocatalytic oxidation of NADH by rutin in biomembrane-like films on glassy carbon electrode

Stable lipid film was made by casting dipalmitoylphosphatidylcholine (DPPC) and rutin onto the surface of a glassy carbon (GC) electrode. The electroc hemical behavior of rutin in the DPPC film was studied. The modified electr ode coated with rutin gave quasi-reversible reduction-oxidation peak on cyc lic voltammogram in the phosphate buffer (pH 7.4). The peak current did not decrease apparently after stored at 4 degreesC for 8 hours in refrigerator . This model of biological membrane was used to investigate the oxidation o f dihydronicotinamide adenine dinucleotide (NADH) by rutin. Rutin in the fi lm acts as a mediator. The modified electrode shows a great enhancement and the anodic peak potential was reduced by about 220 mV in the oxidation of 5 X 10(-3) mol L-1 NADN compared with that obtained at a bare glassy carbon electrode. (C) 2000 Elsevier Science S.A. All rights reserved.