This short communication describes the establishment of a two-dimensional e
lectrophoresis (2-DE) reference map of nuclear proteins isolated from human
liver. The human liver nuclei 2-DE reference map contains 1497 spots. In a
n initial identification study using peptide mass fingerprinting as a means
of protein identification we were able to identify 26 spots corresponding
to 15 different proteins. The human liver nuclei 2-DE reference map is now
included in the SWISS-2DPAGE database, which can be accessed through the Ex
PASy server (http://www.expasy.ch/ch2d/).