Lyn and Syk tyrosine kinases are not activated in B-lineage lymphoid cellsexposed to low-energy electromagnetic fields

Exposure of B-lineage lymphoid cells to a 100 muT 60 Hz AC magnetic field h as been reported to stimulate the rapid activation of Lyn and Syk tyrosine kinases and the induction of protein tyrosine phosphorylation. These findin gs are significant because of the critical role played by these B cell sign aling events in the control of growth and differentiation, and therefore th e potential of electromagnetic field (EMF) exposure to induce cancer. We re port the first study carried out with the aim of reproducing the reported E MF effects on Lyn and Syk tyrosine kinases, The system used enabled EMF exp osure conditions to be carefully controlled and also allowed experiments to be performed blind. The effects of a 100 mur 60 Hz AC magnetic field on pr otein tyrosine phosphorylation and on Lyn and Syk tyrosine kinase activitie s were investigated in Nalm-6 and DT40 B cells in the absence and presence of a 46 muT DC magnetic field. However, no significant effects of low-energ y electromagnetic fields on tyrosine kinase activities or protein phosphory lation were observed.-Woods, M., Bobanovic, F., Brown, D., Alexander, D. R. Lyn and Syk tyrosine kinases are not activated in B-lineage lymphoid cells exposed to low-energy electromagnetic fields.