No evidence of somatic activating mutations on gonadotropin receptor genesin sex cord stromal tumors

Objective: To search for somatic activating mutations of gonadotropin recep tor (FSH-R and LH/chorionic gonadotropin receptor [CG-R]) genes as a cause of sex cord stromal tumors. Design: Molecular studies in human tissue. Setting: University hospital. Specimen(s): Eight granulosa cell tumors collected from paraffin-embedded t issue, eight Leydig cell tumors, and three thecomas collected from fresh-fr ozen or paraffin-embedded tissue. Intervention(s): Tumor samples were used for DNA extraction. The entire exo n 11 of the LH/CG-R gene and a hot spot for gonadotropin receptor activatin g mutations on exon 10 of the FSH-R gene were amplified by polymerase chain reaction. The former was analyzed by denaturing gradient gel electrophores is and automatic direct sequencing, and the latter by automatic direct sequ encing. Main Outcome Measure(s): Results of denaturing gradient gel electrophoresis and automatic direct sequencing. Result(s): No somatic activating mutation was detected in exon 11 of the LH /CG-R gene in eight Leydig cell tumors and three thecomas. In addition, no mutations were detected in eight granulosa cell tumors in the hot spot for activating mutations in exon 10 of the FSH-R gene. Conclusion(s): Somatic activating mutations of gonadotropin receptors seem to Flay no relevant role in the development of sex cord stromal tumors. (C) 2000 by American Society for Reproductive Medicine.