Application of HPLC-NMR coupling using C-30 phase in the separation and identification of flavonoids of taxonomic relevance

The on-line separation and identification of two important taxonomic marker s for plant species of the Paepalanathus Its genus, the flavonoids 6-methox ykaempferol-3-O-beta -D-glucopyranoside and 6-methoxykaempferol-3-O-beta -D -6"(p-coumaroyl)glucopyranoside, has been performed with an HPLC-NMR coupli ng using C-30 phase. 1D spectra have been recorded in the stopped-flow mode for the two predominant chromatographic peaks. This is the first applicati on of HPLC-NMR coupling using C-30 phase to a taxonomic problem. The techni que drastically reduces the required amount of sampling for structure deter mination.