Affinity selection of cDNA libraries by lambda phage surface display

Bacteriophage lambda surface display was used to isolate cDNA clones encodi ng autoantigens recognized by sera from patients with Sjogren's syndrome (S S). We made cDNA libraries from human HeLa and HepG2 cells, using the expre ssion vector lambda foo. By repeating affinity selection of the libraries w ith the sera immobilized in microtiter wells, we isolated three clones that encode previously unknown antigens as well as four clones previously known as SS autoantigens. The newly identified autoantigens include TRK-fused ge ne product (TFG), survival motor neuron gene product (SMN) and pM5, which h as a similarity to the metal-binding domain of human fibroblast collagenase . Thus, the bacteriophage lambda surface display is powerful for isolating cDNA clones by affinity screening. (C) 2000 Elsevier Science B.V. All right s reserved.