Mechanism of inactivation of NF-kappa B by a viral homologue of I kappa B alpha - Signal-induced release of I kappa B alpha results in binding of theviral homologue to NF-kappa B
Swg. Tait et al., Mechanism of inactivation of NF-kappa B by a viral homologue of I kappa B alpha - Signal-induced release of I kappa B alpha results in binding of theviral homologue to NF-kappa B, J BIOL CHEM, 275(44), 2000, pp. 34656-34664
Activation of the nuclear factor kappaB plays a key role in viral pathogene
sis, resulting in inflammation and modulation of the immune response. We ha
ve previously shown that A238L, an open reading frame from African swine fe
ver virus (ASFV), encoding a protein with 40% homology to porcine I kappaB
alpha exerts a potent anti-inflammatory effect in host, macrophages, where
it down-regulates NF-kappaB-dependent gene transcription and proinflammator
y cytokine production. This paper reveals the mechanism of suppression of N
F-kappaB activity by A238Lp. A238Lp is synthesized throughout infection as
two molecular mass forms of 28 and 32 kDa, and vaccinia-mediated expression
of A238L demonstrated that both proteins are produced from a single gene.
Significantly, the higher 32-kDa form of A238L, but not the 28-kDa form, in
teracts directly with RelA, the 65-kDa subunit of NF-kappaB, indicating tha
t the binding is dependent on a posttranslational modification. Immunopreci
pitation analysis shows the NF-kappaB p65-A238L p32 heterodimer is a separa
te complex from NF-kappaB-I kappaB alpha, and it resides in the cytoplasm.
Moreover, we show that ASFV infection stimulates the NF kappaB signal trans
duction pathway, which results in the rapid degradation of endogenous I kap
paB alpha, although both forms of A238Lp are resistant to stimulus-induced
degradation. Using the proteasome inhibitor MG132, we show that when degrad
ation of I kappaB alpha is inhibited, A238Lp binding to NF-kappaB p65 is re
duced. The results suggest that the virus exploits its activation of the NF
-kappaB pathway to enable its own I kappaB homologue to bind to NF-kappaB p
65. Last, we show that synthesis of I kappaB alpha is increased during ASFV
infection, indicating RelA-independent transcription of the I kappaB alpha
gene.