Differential gene expression of rat neonatal heart analyzed by suppressionsubtractive hybridization and expressed sequence tag sequencing

Citation
Ss. Chim et al., Differential gene expression of rat neonatal heart analyzed by suppressionsubtractive hybridization and expressed sequence tag sequencing, J CELL BIOC, 80(1), 2000, pp. 24-36
Citations number
46
Categorie Soggetti
Cell & Developmental Biology
Journal title
JOURNAL OF CELLULAR BIOCHEMISTRY
ISSN journal
07302312 → ACNP
Volume
80
Issue
1
Year of publication
2000
Pages
24 - 36
Database
ISI
SICI code
0730-2312(2000)80:1<24:DGEORN>2.0.ZU;2-I
Abstract
Heart diseases have been one of the major killers among the human populatio n worldwide. Because the vast majority of cardiomyocytes cannot regenerate once they cease to proliferate shortly after birth, functionally significan t: myocardial regeneration is not observed clinically. Whether these cells are terminally differentiated and permanently withdrawn from the cell cycle is controversial, but broadening our understanding of the rapid switch fro m hyperplastic to hypertrophic growth of cardiomyocytes during neonatal myo cardial development may shed light on novel cardiovascular therapies. By su ppression subtractive hybridization (SSH) and expressed sequence tag (EST) sequencing, we analyzed the differential gene expression of rat neonatal he art. SSH yielded subtracted and normalized cDNA libraries and enhanced the probability of detecting ESTs, which represent genes pertinent to signal tr ansduction/cell regulation and replication/transcription/translation machin ery, as compared to the traditional EST sequencing of heart cDNA libraries. (C) 2000 Wiley-Liss, Inc.