Ss. Chim et al., Differential gene expression of rat neonatal heart analyzed by suppressionsubtractive hybridization and expressed sequence tag sequencing, J CELL BIOC, 80(1), 2000, pp. 24-36
Heart diseases have been one of the major killers among the human populatio
n worldwide. Because the vast majority of cardiomyocytes cannot regenerate
once they cease to proliferate shortly after birth, functionally significan
t: myocardial regeneration is not observed clinically. Whether these cells
are terminally differentiated and permanently withdrawn from the cell cycle
is controversial, but broadening our understanding of the rapid switch fro
m hyperplastic to hypertrophic growth of cardiomyocytes during neonatal myo
cardial development may shed light on novel cardiovascular therapies. By su
ppression subtractive hybridization (SSH) and expressed sequence tag (EST)
sequencing, we analyzed the differential gene expression of rat neonatal he
art. SSH yielded subtracted and normalized cDNA libraries and enhanced the
probability of detecting ESTs, which represent genes pertinent to signal tr
ansduction/cell regulation and replication/transcription/translation machin
ery, as compared to the traditional EST sequencing of heart cDNA libraries.
(C) 2000 Wiley-Liss, Inc.