H. Bester et al., Changes in tactile stimuli-induced behavior and c-Fos expression in the superficial dorsal horn and in parabrachial nuclei after sciatic nerve crush, J COMP NEUR, 428(1), 2000, pp. 45-61
Neurons in the superficial laminae of the dorsal horn are dominated by inpu
t from peripheral nociceptors. Following peripheral nerve injury, low thres
hold mechanoreceptive A beta- fibers sprout from their normal termination s
ite in laminae III/IV into laminae I-II and this structural reorganization
may contribute to neuropathic tactile pain hypersensitivity. We have now in
vestigated whether a sciatic nerve crush injury alters the behavioral respo
nse in rats to tactile stimuli and whether this is associated with a change
in the pattern of c-Fos expression in the dorsal horn and the parabrachial
area of the brainstem. Sciatic nerve crush resulted in a patchy but marked
tactile allodynia manifesting first at 3 weeks and persisting for up to 52
weeks. C-Fos expression in the dorsal horn and parabrachial region was nev
er observed on brushing the skin of the sciatic nerve territory in animals
with intact nerves, but was found after sciatic nerve crush with peripheral
regeneration. We conclude that after nerve injury, low threshold mechanore
ceptor fibers may play a major role in producing pain-related behavior by a
ctivating normally nociceptive-specific regions of the central nervous syst
em such as the superficial laminae of the dorsal horn and the parabrachial
area. (C) 2000 Wiley-Liss, Inc.