Production of hydrogen peroxide by Lactobacillus delbrueckii subsp lactis as influenced by media used for propagation of cells

Cells of Lactobacillus delbrueckii subsp. lactis I were grown in several me dia to determine the most suitable medium for production of H2O2 during ref rigerated storage. H2O2 was detected from cells that were resuspended in ph osphate buffer containing variable amounts of glucose. Cells grown in MRS b roth produced more H2O2 during refrigerated storage than those grown in LBS pr PTM. Cells grown in LBS produced more H2O2 then those grown in PTM. For both MIRS and LES, cells inoculated in phosphate buffer containing 0% gluc ose produced significantly more H2O2 than those containing 1% or 10% glucos e. MRS medium appears to be the best medium for growing cells for the produ ction of H2O2.