The importance of high-frequency paced breathing in spectral baroreflex sensitivity assessment

Objective Computation of the low-frequency (LF) blood pressure variability (BPV) to heart rate variability (HRV) transfer-index is a common method to assess baroreflex sensitivity (BRS), tacitly assuming that all LF-HRV is ca used by baroreflex feedback of LF-BPV. However, respiration may also cause HRV by mechanisms not involving the baroreflex, Application of narrow-band (controlled) high-frequency breathing would keep such non-baroreflex-mediat ed HRV best out of the LF band. Spontaneous breathing, because of its broad -band character, might cause extra, non-baroreflex-mediated, HRV in the LF band, while paced LF breathing would even concentrate most non-baroreflex-m ediated HRV in the LF band. Our study addresses the likely resulting BRS ov erestimation. Design We recorded HRV and BPV in 20 healthy young subjects in the sitting position. We varied the sympathovagal balance by gradual leg-lowering from horizontal till 60 degrees. At each angle the subjects performed controlled 0.10 Hz, spontaneous, and controlled 0.25 Hz respiration. Results Resting BRS values were 15.5(7.2), 13.1(3.7), and 11.6(6.2) ms/mmHg , respectively. Both the 15/min and the free breathing values differed sign ificantly, P<0.01 and P = 0.04, from the 6/min breathing value. With lowere d legs, the BRS values were 8.2(3.4), 8.3(2.9), and 8.3(3.4) ms/mmHg, respe ctively. Conclusion Controlled 6/min breathing caused significant BRS overestimation under resting conditions. For the group, spontaneous respiration yielded a cceptable BRS values, but individual BRS values deviated sometimes consider ably. Conversely, with gravitational load, the respiratory pattern had only minor impact on BRS. Our results demonstrate that the risk of an overestim ated BRS value is realistic as long as respiration is not controlled and of high-frequency. J Hypertens 18:1635-1644 (C) 2000 Lippincott Williams & Wi lkins.