Intracellular calcium during fatigue of cane toad skeletal muscle in the absence of glucose

Mechanisms of fatigue were studied in single muscle fibres of the cane toad (Bufo marinus) in which force, intracellular calcium ([Ca2+](i)), [Mg2+](i ), glycogen and the rapidly releasable Ca2+ from the sarcoplasmic reticulum (SR) were measured. Fatigue was produced by repeated tetani continued unti l force had fallen to 50%. Two patterns of fatigue in the absence of glucos e were studied. In the first fatigue run force fell to 50% in 8-10 min. Fat igue runs were then repeated until force fell to 50% in <3 min in the final fatigue run. Addition of extracellular glucose after the final fatigue run prolonged a subsequent fatigue run. In the first fatigue run peak tetanic [Ca2+](i) initially increased and then declined and at the time when force had fallen to 50% tetanic [Ca2+](i) was 54 +/- 5% of initial value. In the final fatigue run force and peak tetanic [Ca2+](i) declined more rapidly bu t to the same level as in first fatigue runs. At the end of the first fatig ue run, the rapidly releasable SR Ca2+ store fell to 46 +/- 6% of the pre-f atigue value. At the end of the final fatigue run the rapidly releasable SR Ca2+ store was 109 +/- 16% of the pre-fatigue value. In unstimulated fibre s the nonwashable glycogen content was 176 +/- 30 mmol glycosyl units/l fib re. After one fatigue run the glycogen content was 117 +/- 17 mmol glycosyl units/l fibre; at the end of the final fatigue run the glycogen content wa s reduced to 85 +/- 9 mmol glycosyl units/l fibre. [Mg2+](i) did not change significantly at the end of fatigue in either the first or the final fatig ue run suggesting that globally-averaged ATP does not decline substantially in either pattern of fatigue. These results suggest that different mechani sms are involved in the decline of tetanic [Ca2+](i) in first compared to f inal fatigue runs. The SR Ca2+ store is reduced in first fatigue runs; this is not the case for the final fatigue run which is associated with a decli ne in glycogen and possibly related to either a non-metabolic effect of gly cogen or a spatially-localised metabolic decline.