Dopamine D2 long receptor-deficient mice display alterations in striatum-dependent functions

The dopamine D2 receptor (D2) system has been implicated in several neurolo gical and psychiatric disorders, such as schizophrenia and Parkinson's dise ase. There are two isoforms of the D2 receptor: the long form (D2L) and the short form (D2S). The two isoforms are generated by alternative splicing o f the same gene and differ only by 29 amino acids in their protein structur es. Little is known about the distinct functions of either D2 isoform, prim arily because selective pharmacological agents are not available. We genera ted D2L receptor-deficient (D2L-/-) mice by making a subtle mutation in the D2 gene. D2L-/- mice (which still express functional D2S) displayed reduce d levels of locomotion and rearing behavior. Interestingly, haloperidol pro duced significantly less catalepsy and inhibition of locomotor activity in D2L-/- mice. These findings suggest that D2L and D2S may contribute differe ntially to the regulation of certain motor functions and to the induction o f the extrapyramidal side effects associated with the use of typical antips ychotic drugs (e.g., haloperidol). Quinpirole induced a similar initial sup pression of locomotor activity in both D2L-/- and wild-type mice. In additi on, the D2S receptor in the mutant mice functioned approximately equally we ll as did D2L as an impulse-modulating autoreceptor. This suggests that the functions of these two isoforms are not dependent on the formation of rece ptor heterodimers. Our findings may provide novel information for potential ly developing improved antipsychotic drugs.