GM-CSF-DEPENDENT AND IL-3-DEPENDENT CD34 EXPRESSING MYELOID CELL-LINE(SAS-1) ESTABLISHED FROM CD7 AND CD34 EXPRESSING ACUTE MYELOBLASTIC LEUKEMIC-CELLS

A novel factor-dependent human myeloid leukemia cell line (SAS-I) was established from a 69-year-old Japanese male suffering from CD7 and CD 34 expressing acute myeloblastic leukemia (AML M2 in FAB classificatio n). Morphological and cytochemical staining showed that SAS-I cells we re round with basophilic cytoplasm which is positive for peroxidase. A nalysis of surface markers revealed that SAS-1 cells were myeloblasts derived from an immature progenitor origin, which express CD34. The co nsensus karyotype of the cell line was 41 XY 5q-, -7,11p-, 12p+, -13, -14, -16, -17, -19, -22, with two markers. The proliferation of SAS-I cells was dependent on the presence of either granulocyte-macrophage c olony-stimulating factor (GM-CSF) or interleukin-3 (IL-3), and GM-CSF- and IL-3-induced proliferation was dose dependent. Neither, stem cell factor (SCF) nor granulocyte colony-stimulating factor (G-CSF) alone supported the growth of SAS-1 cells, but supported their viability for more than 4 days and arrested them in the G0/G1 phase. SCF also enhan ced GM-CSF- or IL-3-induced growth, but other cytokines did not have t his synergistic effect. Clonogenic assays revealed that SAS-1 cells fo rmed 36.0+/-5.7 or 41.5+/-0.7 colonies/1000 cells in the presence of G M-CSF or IL-3, respectively. SCF also increased the number of colonies formed by GM-CSF or IL-3 treatment, while SAS-1 cells did not form co lonies in the presence of SCF alone. SAS-I cells may prove to be a use ful tool for studying the regulation of the cell cycle, myeloid prolif eration, and differentiation. (C) 1997 Elsevier Science Ireland Ltd.