A simple technique for rapid colonization of Anopheles quadrimaculatus using adults aspirated from livestock barns

A technique was developed for rapid colonization of Anopheles quadrimaculat us Larvae in an improvised insectary using blood-fed mosquitoes aspirated f rom livestock barns. A novel device termed the mosquito aspiration transfer and ovipositional chamber (MATOC) is described. In 2 field seasons, 14 bro ods were successfully mass reared, yielding more than 28,500 vigorous 3rd- and 4th-stage larvae used in rice plot and other bioassays. Crowding the fe males over a natural ovipositional substrate induced oviposition as early a s 12 h from introduction into the MATOCs.