Adenovirus-mediated gene transfer of human interleukin 10 ameliorates reperfusion injury of rat lung isografts

Objective: The objective of this study was to examine the feasibility of hu man interleukin 10 gene transfer into rat lung isografts and to investigate the effect of gene transfer on subsequent ischemia-reperfusion injury. Methods: Male F344 rats were divided into 4 groups and underwent left lung isotransplantation. Twenty-four hours before harvest, 5 x 10E9 pfu (group I , n = 6) or 1 x 10E10 pfu (group II, n = 7) of AdRSVhIL-10 was intravenousl y administered to donor rats. In group I-C (n = 6) and group II-C (n = 6), serving as controls, 5 x 10E9 pfu and 1 x 10E10 pfu of AdCMVLacZ were admin istered, respectively. Grafts were preserved for 18 hems at 4 degreesC befo re implantation and assessed 24 hours after reperfusion. Transgene expressi on of human interleukin 10 was assessed by both reverse transcriptase-polym erase chain reaction and immunohistochemistry. Graft inducible nitric oxide synthase, tumor necrosis factor alpha, intercellular adhesion molecule-1, growth-regulated gene product/cytokine-induced neutrophil chemoattractant-l , and monocyte chemotactic protein-1 mRNA expression were assessed by rever se transcriptase-polymerase chain reaction. Isograft gas exchange, exhaled nitric oxide, and myeloperoxidase activity were also analyzed. Results: Dose-dependent transgene expression was detected by reverse transc riptase-polymerase chain reaction and immunohistochemistry. Arterial Po-2 i n groups I (164.72 +/- 85.3 mm Hg) and II (153.19 +/- 113 mm Hg) was signif icantly higher than in groups I-C (82.37 +/- 19.1 mm Hg) and II-C (77.95 +/ - 33.4 mm HE) (P = .022 and P = .031, respectively). Arterial PCO2 in group I (33.40 +/- 6.80 mm Hg) was significantly lower than in group I-C (51.23 +/- 11.9 mm Hg) (P = .0096). Myeloperoxidase activity in group II (0.083 +/ - 0.031 Delta OD.min(-1).mg(-1)) was significantly lower than in group II-C (0.117 +/- 0.028 Delta OD.min(-1).mg(-1)) (P = .044). The inducible nitric oxide synthase mRNA expression in group TT (0.627 +/- 0.28) was significan tly lower than in group II-C (1.125 +/- 0.63) (P = .039). Conclusion: Adenovirus-mediated human interleukin 10 gene transfer in vivo into lung isografts ameliorates subsequent ischemia-reperfusion injury. Thi s results in improved graft gas exchange, reduced neutrophil sequestration, and down-regulation of graft inducible nitric oxide synthase mRNA expressi on.