DETECTION OF PHOSPHATIDYLCHOLINE HYDROPEROXIDE PRODUCED IN THE HEART OF THE DOXORUBICIN ADMINISTERED MOUSE

Authors
KUMAKURA K KITADA M HORIE T AWAZU S
Citation
K. Kumakura et al., DETECTION OF PHOSPHATIDYLCHOLINE HYDROPEROXIDE PRODUCED IN THE HEART OF THE DOXORUBICIN ADMINISTERED MOUSE, Analytical letters, 30(8), 1997, pp. 1483-1490
Citations number
14
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical letters → ACNP
ISSN journal
00032719
Volume
30
Issue
8
Year of publication
1997
Pages
1483 - 1490
Database
ISI
SICI code
0003-2719(1997)30:8<1483:DOPHPI>2.0.ZU;2-W
Abstract
A chemiluminescence(CL)-high performance liquid chromatography (HPLC) system, where CL detector was equipped with normal phase HPLC, was use d in order to specifically detect lipid hydroperoxide in the heart of doxorubicin (DXR) administered mice. Luminol and cytochrome c were use d as the luminescence reagents specific for the hydroperoxide. Phospha tidylcholine hydroperoxide was detected in the heart, indicating parti cipation of lipid peroxidation in the DXR-induced cardiotoxicity. This system is of great use to study the DXR-induced lipid peroxidation in vivo.