B. Laassis et al., FLUOROMETRIC AND PHOTOCHEMICALLY INDUCED FLUOROMETRIC-DETERMINATION OF ETHOPROPAZINE, LEVOMEPROMAZINE, THIOPROPERAZINE AND TRIFLUOPERAZINE, Analytical letters, 30(8), 1997, pp. 1541-1554
Fluorimetry and photochemically induced fluorimetry (PIF) are proposed
for the quantitative analysis of four psychotropic drugs, including e
thopropazine (EPZ), levomepromazine (LPZ), thioproperazine (TPZ) and t
rifluoperazine (TFZ). The influence of ultraviolet (UV) irradiation ti
me on the fluorescence excitation and emission wavelengths and intensi
ty was investigated in aqueous buffer solutions (pH 4 - 6) at room tem
perature (298 K). The selected UV irradiation times were between 10 an
d 50 s, depending on the compound. Linear calibration graphs were esta
blished over about two to four orders of magnitude, with relative stan
dard deviations in the range 2.0 - 5.4%. PIF limits of detection were
comprised between 2.6 and 8 ng ml(-1), depending on the compound. The
PIF method was found to be more sensitive and more reliable than conve
ntional fluorimetry for the phenothiazine derivatives under study. PIF
was applied to the determination of these compounds in urine rind pha
rmaceutical samples. The mean recoveries ranged from 88 to 119%.