Determination of pH using water protons and chemical exchange dependent saturation transfer (CEST)

Authors
Ward, KM Balaban, RS
Citation
Km. Ward et Rs. Balaban, Determination of pH using water protons and chemical exchange dependent saturation transfer (CEST), MAGN RES M, 44(5), 2000, pp. 799-802
Citations number
16
Categorie Soggetti
Radiology ,Nuclear Medicine & Imaging","Medical Research Diagnosis & Treatment
Journal title
MAGNETIC RESONANCE IN MEDICINE
ISSN journal
07403194 → ACNP
Volume
44
Issue
5
Year of publication
2000
Pages
799 - 802
Database
ISI
SICI code
0740-3194(200011)44:5<799:DOPUWP>2.0.ZU;2-T
Abstract
Solution pH was measured using water proton NMR via chemical exchange depen dent saturation transfer (CEST) with selected chemical exchange sites. Seve ral useful pH-sensitive proton chemical exchange agents were found: 5,6-dih ydrouracil, 5-hydroxytryptophan, and a combination of 5-hydroxytryptophan a nd 2-imidazolidinethione. A ratiometric approach was developed that permitt ed pH determinations that were independent of water T-1 or exchange site co ncentration. Published 2000 Wiley-Liss, Inc.(dagger)