Molecular analysis of the folC gene of Pseudomonas aeruginosa

We have cloned the Pseudomonas aeruginosa folC gene coding for folylpolyglu tamate synthetase-dihydrofolate synthetase, which was located between the t rpF and purF loci, and determined the nucleotide sequence of the folC gene and its flanking region. The deduced amino acid sequence of P, aeruginosa F olC was highly homologous to that of Escherichia coli FolC, The cloned gene complemented E. coli folC mutations and was found to encode both folylpoly glutamate synthetase and dihydrofolate synthetase activities. The gene orga nization around the folC gene in P, aeruginosa was completely conserved wit h that in E. coli; the accD gene was located upstream of the folC gene, and dedD, cvpA and purF genes followed the folC gene in this order. The gene a rrangement and the result of the promoter activity assay suggested that the P. aeruginosa accD and folC genes were co-transcribed.