O. Levillain et al., Ornithine metabolism along the female mouse nephron: localization of ornithine decarboxylase and ornithine aminotransferase, PFLUG ARCH, 440(5), 2000, pp. 761-769
The fide of ornithine in the nephron of the female OF-1 Swiss mouse remains
unknown. The aim of the present study was to identify the nephron segments
containing the key enzymes involved in ornithine metabolism: ornithine dec
arboxylase (ODC) and ornithine aminotransferase (OAT). Viable tubules isola
ted by microdissection were incubated with [1-C-14]ornithine to study the o
xidative pathway. Other tubules were permeabilized to measure the ODC activ
ity. Ornithine was de carboxylated in all intact tubules, Gabaculine, a sui
cide inhibitor of OAT, and rotenone sharply decreased the production of (CO
2)-C-14 from [1-C-14]ornithine. No ODC activity was round in permeabilized
tubules isolated from untreated mice. Testosterone increased ODC activity i
n the proximal tubule substantially and to a minor extent in other nephron
segments. In situ hybridization showed ODC messenger ribonucleic acid (mRNA
) to be absent in kidneys of untreated females but abundant in the cortex a
nd the outer stripe of the outer medulla of testosterone-treated female mic
e. The whole proximal tubule contained a great density of silver grains cor
responding to ODC mRNA. In conclusion, no basal ODC activity was found in t
he nephron of female mice. The testosterone-inducible ODC is localized main
ly in the proximal tubule, but is also present in distal tubules and collec
ting ducts. OAT is distributed along the whole nephron, but its activity is
higher in proximal tubules than in distal tubules.