Flavonol glycosides from Aristeguietia discolor reduce morphine withdrawalin vitro

The effects of extracts, partially purified fractions and four flavonol gly cosides 1-4 from Aristeguietia discolor were investigated on the naloxone-p recipitated withdrawal contraction of the acute morphine dependent guinea-p ig ileum in vitro. After a 4 min in vitro exposure to morphine a strong con traction of guinea-pig isolated ileum was observed after the addition of na loxone, Both MeOH extract (50, 100 and 200 mg/mL), the partially purified f ractions I, L, M and N (50, 100 and 200 mg/mL) and flavonol glycosides 1-4 (1 x 10(-4) 5 x 10(-5) 1 x 10(-5) M), injected 10 min before morphine, were capable of blocking the naloxone-induced contraction after exposure to mor phine in a concentration-dependent fashion. The results of the present paper suggest that flavonol glycosides from Aris teguietia discolor may play an important role in the control of morphine wi thdrawal. Copyright (C) 2000 John Wiley & Sons, Ltd.