The paper presents integrated measurements of N fixation, net mineralisatio
n, pasture yield and change in soil mineral N over a 12 month period for da
iry pastures on a sandy loam soil in the South East of South Australia. The
two adjacent pastures studied were an irrigated perennial white clover-rye
grass and an annual non-irrigated subterranean clover with mixed annual gra
sses. This produced the most comprehensive mineral N balance reported for g
razed pastures, to the authors' knowledge, allowing calculation of gaseous
and leaching losses of N (210 kg ha(-1) in the irrigated and paddock and 81
kg ha(-1) in the non irrigated paddock) primarily from urine patches. In b
oth paddocks these losses were about three times the N yield in milk (61 an
d 28 kg N ha(-1) respectively) and were replenished by biological N fixatio
n (294 and 100 kg N ha(-1)). However, mineralisation of soil organic N, exc
retal N and pasture residues (687 and 438 kg N ha(-1)) was the major source
of mineral N for cycling and losses. The results demonstrate the enormous
impact of pasture management on N fluxes and reinforce the importance of li
vestock urine on the magnitude of N fluxes including gaseous and leaching l
osses.