New lanostanoids from Ganoderma lucidum that induce NAD(P)H : quinone oxidoreductase in cultured hepalclc7 murine hepatoma cells

Two new lanostanoids were isolated from the basidiocarp of Ganoderma lucidu m and were identified as 26,27-dihydroxy-5 alpha -lanosta-7,9(11),24-triene -3,22-dione (1) and 26-hydroxy-5 alpha -lanosta-7,9(11),24-triene-3,22-dion e (2) by their respective spectral data. Crude extracts and the isolated co mpounds were tested for their potential to induce NAD(P)H:quinone oxidoredu ctase (QR), a phase 2 drug-metabolizing enzyme, as an approach to detect po tential cancer chemopreventive activity. Compound 2 doubled the specific ac tivity of QR at a concentration of 3.0 mug/ml, whereas compound 1 was signi ficantly less active (1.7-fold induction at 20 mug/ml). In addition, both c ompounds weakly inhibited sheep vesicle cyclooxygenase 1 activity at a test concentration of 40 mug/ml.